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The ceramic hydrocyclone VF2 S2 was scanned, 3D printed and used for comparison. The scanned hydrocyclone has a diameter of 10 mm, a cylindrical body height of 2 mm and a vortex finder length of 6 mm. It has a tangential square inlet of 4 mm 2 with a downward guided-channel. The conical section has a height of 54 mm and a cone …
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The small 10 cm – high Cell Culture Hydrocyclone has a maximum process-specific perfusion performance of 450–720 liters of cell culture harvest per day while operating …
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However, harvesting the microalgal cells from its culture medium is a huge bottleneck for its widespread commercialization. Harvesting of microalgae includes separation of the microalgal solid cells from the dilute algal suspension of 0.02–0.05% dry solids concentration. ... Hydrocyclone. Hydrocyclone is a cylindrical hopper-type …
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pumped through the mini-hydrocyclone, cells exit through an overflow or underflow port depending ... the flocculation-based technique for algal cell harvesting is more convenient because a large ...
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The small 10 cm – high Cell Culture Hydrocyclone has a maximum process-specific perfusion performance of 450–720 liters of cell culture harvest per day while operating at a flow rate of 0.3 ...
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A continuous Chinese hamster ovary (CHO) cell culture process comprised of a highly proliferative N-1 perfusion bioreactor utilizing a hydrocyclone as a cell retention device linked to a production continuous-flow stirred tank reactor (CSTR) is presented. The overflow stream from the hydrocyclone, w …
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The schematic of the hydrocyclone membrane filtration cell (HMFC) developed in this study is illustrated in Fig. 1 a. The cell was produced by a stereo lithography appearance method with a photosensitive resin and comprised three separable parts: (i) an upper cylinder with a vortex and a feed inlet, (ii) a middle cyclone …
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2.1.3.4 Hydrocyclone Cell Retention. A hydrocyclone is a simple conical device that uses centrifugal forces to separate components by density. Figure 2 depicts this separator in a perfusion application: cell culture enters at high speeds and circles the cone.
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Gregg et al. [138] reported a low harvesting efficiency for particles smaller than 400 μm using hydrocyclones for the harvesting of green algae. In terms of cell damage, a hydrocyclone is predicted to cause a certain level of mechanical disruption, however, there is a lack of specialized studies on its effect for large colonial microalgae …
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hydrocyclone mammalian cell retention device for and reusable bioreactors perfusion technologies overflow (harvest) feed feed product specification material. ... prior to harvest due to long residence times in. the bioreactor. Perfusion mode is an alterna-tive process operation methodology which.
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This review highlights the potential of the application of hydrocyclones for mammalian cell separation in continuous perfusion biorecators and focuses on the latest developments …
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Fig. 1 demonstrates the mini-hydrocyclone geometric profile and Table 1 lists the specific parameter values. The parameter n is referred to as the conical shape factor and is defined by Eq. (1) [43], as given below, (1) L − Z L − H = 2 X − D U D C − D U n where the coordinate origin is located at the center of the spigot outlet, as shown in Fig. …
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DOI: 10.6000/1927-3037.2013.02.04.2 Corpus ID: 84350518; The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors @article{Elsayed2013ThePO, title={The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors}, author={Elsayed …
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This article is published in International Journal of Biotechnology for Wellness Industries.The article was published on and is currently open access. It has received 1 citation(s) till now. The article focuses on the topic(s): Hydrocyclone.
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In our previous study, we proposed a 3D-printed micro-hydrocyclone that could be applied to the primary harvesting of microalgae cells. 31 In that study, however, the flow inside the micro-hydrocyclone could not be observed due to the opaque materials used in the device. However, we realized that without being able to visualize the particle ...
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A novel hydrocyclone membrane filtration cell was constructed by 3D .. CFD simulation indicates strong swirling turbulent flow with high TKE and shear strain.. The resulting hydrodynamic particle transport in HMFC were systematically analyzed.. Fouling eliminated by particle grading, revolving, rotation and turbulent …
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To showcase its utility, high-throughput algae harvesting from the medium with low energy input is demonstrated for the marine microalgae Tetraselmis suecica. Final microalgal biomass concentration was increased by 7.13 times in 11 minutes of operation time using our designed hydrocyclone (HC-1).
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In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells. The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing size in the ...
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The small 10 cm – high Cell Culture Hydrocyclone has a maximum process-specific perfusion performance of 450–720 liters of cell culture harvest per day while operating …
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Such investigation has demonstrated that hydrocyclones are promising in separating mammalian cells, with cell separation efficiency at 81 % for one hydrocyclone (94% …
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Syed et al. [16] applied a 3D-printed mini-hydrocyclone for microalga harvesting and found that a reduction in the hydrocyclone size resulted in a smaller cut-size of particles. Kyriakidis et al. [17] compared 25 types of designs via regression techniques and investigated the effects of underflow orifice diameter and vortex finder …
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To the best of authors' knowledge, although the first patent on the hydrocyclone is about 128 years old, to date, there are just a few reviews on hydrocyclones (Table 1), and marginal effort has been attempted to comprehensively review and summarize enhanced-separation hydrocyclone technologies developed by …
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Hydrocyclone can also be operated continuously with a low maintenance requirement; ... paramylon production, and efficient cell harvesting. KEY POINTS: • Euglena gracilis is an edible microalga ...
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Hence, the results indicate that the hydrocyclone can be appropriately used for cell retention and separation in perfusion cultures. Application at higher pressures is recommended …
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With increasing the length of the cylinder body of the hydrocyclone from 0.4 to 1 m, and the length of the conical section from 1 to 1.7 m, the particle separation efficiency increased by an average of 3.4% and 10.3% respectively. Hydrocyclone with tangential inlet had an average of 6.6% less efficiency than one from Sarcheshmeh …
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Combining harvesting with cell lysis can be desirable to allow for the extraction of lipids [79]. However, there is a significant additional cost associated with using ozone compared to ambient air for the flotation process. ... The microspheres used in this process can then be recovered using a hydrocyclone and reused [54], [80].
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We review the basic design, operation, and features of typical methods and equipment used in (e.g. fed-batch) industrial-scale cell culture harvest, as well as highlight some …
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The small 10 cm – high Cell Culture Hydrocyclone has a maximum process-specific perfusion performance of 450–720 liters of cell culture harvest per day while operating at a flow rate of 0.3 ...
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A 3D-printed mini-hydrocyclone for high throughput particle separation: application to primary harvesting of microalgae ... as well as experimentation of 3D-printed miniaturized hydrocyclones with smaller cut-size for high-throughput particle/cell sorting. The characteristics of the mini-cyclones were numerically investigated using ...
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The reasons behind the high cost of harvesting are due to dilute algae solutions, the large volumes to be processed, and the small size of microalgal cells [34,35]. Hence, determining the most suitable harvesting technique is dependent on the properties of microalgae, such as size, and characteristics of algal strain like cell density [[36 ...
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